Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene. The efficiency scores will instead be shown as '-'. Parental bacterial expression vector for TOPO® TA cloning of PCR products. Plasmids are created by SnapGene program. In other words, some guides may be fine, the problem may just be that the on-target is shown as an off-target.īecause there is no flanking sequence available, the guides in your sequence that are within 50bp of the ends will have no efficiency scores. PER1, PER2, CRY1, and CRY2 which are translated products of PER and CRY genes translocate into the nucleus. In this case, the specificity scores of guide sequences are too low.
#PER2 SNAPGENE FOR FREE#
Sequence Author: MilliporeSigma (Novagen) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.
#PER2 SNAPGENE SOFTWARE#
When reading the list of guide sequences and off-targets below, bear in mind that in case that the input sequence is really in the genome and just has a few differences, the software will use the first found match as the on-target as it cannot distinguish 0-mismatch off-targets from 0-mismatch on-targets. Bacterial vector that encodes a signal sequence for expression of proteins in the periplasm. positive regulators and drive the expression of the negative regulators CRY1 and CRY2, as well as PER1, PER2, and PER3. Diseases associated with PER2 include Advanced Sleep Phase Syndrome, Familial, 1 and Advanced Sleep Phase Syndrome. Use a tool like BLAT to check if the sequence really has a 100% identical match in the target genome. PER2 (Period Circadian Regulator 2) is a Protein Coding gene. If not, you might want to check if you selected the right genome for your query sequence. Warning: The query sequence was not found in the selected genome.
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